Domestic Animal Endocrinology
Volume 24, Issue 1 , Pages 31-41 , January 2003

Development and validation of a specific radioimmunoassay for equine osteocalcin

  • Bianca Carstanjen

      Affiliations

    • Department of Large Animal Internal Medicine, Faculty of Veterinary Medicine, University of Liège, Bd de Colonster No. 20, B 42, Sart Tilman, 4000 Liège, Belgium
    • ,
  • José Sulon

      Affiliations

    • Department of Physiology of Reproduction, Faculty of Veterinary Medicine, University of Liège, Bd de Colonster No. 20, B 41, Sart Tilman, 4000 Liège, Belgium
    • ,
  • Henri Banga-Mboko

      Affiliations

    • Department of Physiology of Reproduction, Faculty of Veterinary Medicine, University of Liège, Bd de Colonster No. 20, B 41, Sart Tilman, 4000 Liège, Belgium
    • ,
  • Jean François Beckers

      Affiliations

    • Department of Physiology of Reproduction, Faculty of Veterinary Medicine, University of Liège, Bd de Colonster No. 20, B 41, Sart Tilman, 4000 Liège, Belgium
    • ,
  • Benoit Remy

      Affiliations

    • Department of Physiology of Reproduction, Faculty of Veterinary Medicine, University of Liège, Bd de Colonster No. 20, B 41, Sart Tilman, 4000 Liège, Belgium
    • Corresponding Author InformationCorresponding author. Tel.: +32-4-366-4168; fax: +32-4-366-4165.

Received 10 April 2002 ,Accepted 24 June 2002.

References 

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  2. Camarda AJ, Butler WT, Finkelman RD, Nanci A. Immunocytochemical localization of gamma-carboxyglutamic acid-containing proteins (osteocalcin) in rat bone and dentin. Calcif. Tissue Int. 1987;40:349–355
  3. Hauschka PV, Reid ML. Vitamin K dependence of a calcium-binding protein containing gamma-carboxyglutamic acid in chicken bone. J. Biol. Chem. 1978;253:9063–9068
  4. Lian J, Stewart C, Puchacz E, Mackowiak S, Shalhoub V, Collart D, et al. Structure of the rat osteocalcin gene and regulation of Vitamin D-dependent expression. Proc. Natl. Acad. Sci. U.S.A. 1989;86:1143–1147
  5. Carstanjen B, Wattiez R, Amory H, Lepage OM, Remy B. Isolement et caractérisation de l’ostéocalcine équine. Ann. Méd. Vét. 2002;146:31–38
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  7. Price PA, Nishimoto SK. Radioimmunoassay for the Vitamin K-dependent protein of bone and its discovery in plasma. Proc. Natl. Acad. Sci. U.S.A. 1980;77:2234–2238
  8. Price PA, Williamson MK, Lothringer JW. Origin of a Vitamin-K dependent bone protein found in plasma and its clearance by kidney and bone. J. Biol. Chem. 1981;256:12760–12766
  9. Farrugia W, Melick RA. Metabolism of osteocalcin. Calcif. Tissue Int. 1986;39:234–238
  10. Patterson-Allen P, Brautigam CE, Grindeland RE, Asling CW, Callahan PX. A specific radioimmunoassay for osteocalcin with advantageous species crossreactivity. Anal. Biochem. 1982;120:1–7
  11. Garnero P, Grimaux M, Seguin P, Delmas PD. Characterization of immunoreactive forms of human osteocalcin generated in vivo and in vitro. J. Bone Miner. Res. 1994;9:255–264
  12. Price PA, Parthemore JG, Deftos LJ. New biochemical marker for bone metabolism. Measurement by radioimmunoassay of bone GLA protein in the plasma of normal subjects and patients with bone disease. J. Clin. Invest. 1980;66:878–883
  13. Delmas PD, Wahner HW, Mann KG, Riggs BL. Assessment of bone turnover in postmenopausal osteoporosis of bone Gla protein. J. Lab. Clin. Med. 1983;102:470–476
  14. Delmas PD, Stenner D, Wahner HW, Mann KG, Riggs BL. Increase in serum bone gamma-carboxyglutamic acid protein with aging in women. Implications for the mechanism of age-related bone loss. J. Clin. Invest. 1983;71:1316–1321
  15. Malluche HH, Faugere MC, Fanti P, Price PA. Plasma levels of bone Gla-protein reflect bone formation in patients on chronic maintenance dialysis. Kidney Int. 1984;26:869–874
  16. Risteli L, Risteli J. Biochemical markers of bone metabolism. Ann. Med. 1993;25:385–393
  17. Mäenpää PE, Pirskanen A, Koskinen E. Biochemical indicators of bone formation in foals after transfer from pasture to stables for winter months. Am. J. Vet. Res. 1988;49:1990–1992
  18. Black A, Schoknecht PA, Ralston SL, Shapses SA. Diurnal variation and age differences in the biochemical markers of bone turnover in horses. J. Anim. Sci. 1999;77:75–83
  19. Lepage OM, Eicher R, Übelhart B, Tschudi P. Influence of type and breed of horse on serum osteocalcin concentration, and evaluation of the applicability of a bovine radioimmunoassay and a human immunoradiometric assay. Am. J. Vet. Res. 1997;58:574–578
  20. Colombo G, Fanti P, Yao C, Malluche HH. Isolation and complete amino acid sequence of osteocalcin from canine bone. J. Bone Miner. Res. 1993;8:733–743
  21. Vaitukaitis J, Robbins JB, Nieschlag E, Ross GT. A method for producing specific antisera with small doses of immunogen. J. Clin. Endocrinol. Metab. 1971;33:988–991
  22. Price PA, Otsuka AS, Poser JW, Kristaponis J, Raman N. Characterization of a γ-carboxyglutamic acid-containing protein from bone. Proc. Natl. Acad. Sci. U.S.A. 1976;73:1447–1451
  23. Lepage OM, Marcoux M, Tremblay A. Serum osteocalcin or bone Gla-protein, a biochemical marker for bone metabolism in horses: differences in serum levels with age. Can. J. Vet. Res. 1990;54:223–226
  24. Delmas PD, Wilson DM, Mann KG, Riggs BL. Effect of renal function on plasma levels of bone Gla-protein. J. Clin. Endocrinol. Metab. 1983;57:1028–1030
  25. Hope E, Johnston SD, Hegstad RL, Geor RJ, Murphy MJ. Effects of sample collection and handling on concentration of osteocalcin in equine serum. Am. J. Vet. Res. 1993;54:1017–1020
  26. Bouillon R, Vanderschueren D, Van Herck E, Nielsen HK, Bex M, Heyns W, et al. Homologous radioimmunoassay of human osteocalcin. Clin. Chem. 1992;38:2055–2060
  27. Delmas PD, Price PA, Mann KG. Validation of the bone Gla protein (osteocalcin) assay. J. Bone Miner. Res. 1990;5:3–4
  28. Taylor AK, Linkhart SG, Mohan S, Baylink DJ. Development of a new radioimmunoassay for human osteocalcin: evidence for a midmolecule epitope. Metabolism. 1988;37:872–877
  29. Hosoda K, Eguchi H, Nakamoto T, Kubota T, Honda H, Jindai S, et al. Sandwich immunoassay for intact human osteocalcin. Clin. Chem. 1992;38:2233–2238

PII: S0739-7240(02)00185-6

Domestic Animal Endocrinology
Volume 24, Issue 1 , Pages 31-41 , January 2003

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