Domestic Animal Endocrinology
Volume 31, Issue 2 , Pages 173-185 , August 2006

Production and characterization of recombinant equine prorelaxin

  • Jennifer L. Neumann

      Affiliations

    • Department of Animal Sciences, Rutgers University, 84 Lipman Drive, New Brunswick, NJ 08901, USA
  • ,
  • Anthoula Lazaris

      Affiliations

    • Nexia Biotechnologies, Inc., Vaudreuil-Dorion, Que., Canada J7V 8P5
  • ,
  • Yue-Jin Huang

      Affiliations

    • Nexia Biotechnologies, Inc., Vaudreuil-Dorion, Que., Canada J7V 8P5
  • ,
  • Costas Karatzas

      Affiliations

    • Nexia Biotechnologies, Inc., Vaudreuil-Dorion, Que., Canada J7V 8P5
  • ,
  • Peter L. Ryan

      Affiliations

    • Department of Animal and Dairy Sciences, Mississippi State University, Mississippi State, MS 39762, USA
  • ,
  • Carol A. Bagnell

      Affiliations

    • Department of Animal Sciences, Rutgers University, 84 Lipman Drive, New Brunswick, NJ 08901, USA
    • Corresponding Author InformationCorresponding author. Tel.: +1 732 932 0535; fax: +1 732 932 6996.

Received 21 June 2005 ,Revised 23 September 2005 ,Accepted 5 October 2005.

References 

  1. Sherwood OD. Relaxin. In:  Knobil E,  Neill J editor. The physiology of reproduction. 2nd ed.. New York: Raven Press; 1994;p. 861–1009
  2. Ryan PL, Klonisch T, Tamashiro S, Renaud RL, Wasnidge C, Porter DG. Expression and localization of relaxin in the ovary of the mare. J Reprod Fertil. 1997;110:329–338
  3. Stewart DR, Stabenfeldt GH, Hughes JP, Meagher DM. Determination of the source of equine relaxin. Biol Reprod. 1982;27:17–24
  4. Stewart DR, Stabenfeldt GH. Relaxin activity in the pregnant mare. Biol Reprod. 1981;25:281–289
  5. Klonisch T, Mathias S, Cambridge G, Hombach-Klonisch S, Ryan PL, Allen WR. Placental localization of relaxin in the pregnant mare. Placenta. 1997;18:121–128
  6. Stewart DR, Addiego LA, Pascoe DR, Haluska GJ, Pashen R. Breed differences in circulating equine relaxin. Biol Reprod. 1992;46:648–652
  7. Ryan PL, Vaala WE, Bagnell CA. Evidence that equine relaxin is a good indicator of placental insufficiency in the mare. In: American Association of Equine Practitioners Proceedings, vol. 44. 1998;p. 62–63
  8. Yang S, Heyn H, Zhang YZ, Bullesbach EE, Schwabe C. The expression of human relaxin in yeast. Arch Biochem Biophys. 1993;300:734–737
  9. Vandlen R, Winslow J, Moffat B, Rinderknecht E. Human relaxin: purification, characterization and production of recombinant relaxins for structure function studies. In:  MacLennan AH,  Tregear GW,  Bryant-Greenwood GD editor. Progress in relaxin research. Singapore: Global Publication Services; 1995;p. 59–72
  10. Silvertown JD, Geddes BJ, Summerlee AJS. Adenovirus-mediated expression of human prorelaxin promotes the invasive potential of canine mammary cancer cells. Endocrinology. 2003;144:3683–3691
  11. Vu AL, Green CB, Roby KF, Soares MJ, Fei DTW, Chen AB, et al. Recombinant porcine prorelaxin produces in Chinese hamster ovary cells is biologically active. Life Sci. 1993;52:1055–1061
  12. Stewart AG, Richards H, Roberts S, Warwick J, Edwards K, Bell L, et al. Cloning and expression of a porcine prorelaxin gene in E. coli. Nucleic Acids Res. 1983;11:6597–6609
  13. Reddy GK, Gunwar S, Green CB, Fei DTW, Chen AB, Kwok SCM. Purification and characterization of recombinant porcine prorelaxin expressed in Escherichia coli. Arch Biochem Biophys. 1992;294:579–585
  14. Zarreh-Hoshyari-Khah R, Bartsch O, Einspanier A, Pohnke Y, Ivell R. Bioactivity of recombinant prorelaxin from the marmoset monkey. Regul Pept. 2001;97:139–146
  15. Huyuh HT, Robitaille G, Turner JD. Establishment of bovine mammary epithelial cells (MAC-T): an in vitro model for bovine lactation. Exp Cell Res. 1991;197:191–199
  16. Lazaris A, Arcidiacono S, Huang Y, Zhou J-F, Duguay F, Chretien N, et al. Spider silk fibers spun from soluble recombinant silk produced in mammalian cells. Science. 2002;295:472–476
  17. Min K-S, Shiota K, Ogawa T. Molecular cloning of equine preprorelaxin cDNA. J Reprod Dev. 1996;42:171–178
  18. Felgner PL, Gadek TR, Holm M, Roman R, Chan HW, Wenz M, et al. Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure. Proc Natl Acad Sci USA. 1987;84:7413–7417
  19. Stewart DR. Development of a homologous equine relaxin radioimmunoassay. Endocrinology. 1986;119:1100–1104
  20. Tsuchiya S, Yamabe M, Yamaguchi Y, Kobayaski Y, Konno T, Tada K. Establishment and characterization of a human monocytic-leukemic cell line (THP-1). Int J Cancer. 1980;26:171–176
  21. Parsell DA, Mak JY, Amento EP, Unemori E. Relaxin binds to and elicits a response from cells of the human monocytic cell line THP-1. J Biol Chem. 1996;27:27936–27941
  22. Soloff MS, Shaw AR, Gentry LE, Marquadt H, Vasilenko P. Demonstration of relaxin precursors in pregnant rat ovaries with antisera against bacterially expressed rat prorelaxin. Endocrinology. 1992;130:1844–1851
  23. Stewart DR, Nevins B, Hadas E, Vandlen R. Affinity purification and sequence determination of equine relaxin. Endocrinology. 1991;129:375–383
  24. Kwok SMC, Chamley WA, Bryant-Greenwood GD. High molecular weight forms of relaxin in pregnant sow ovaries. Biochem Biophys Res Commun. 1978;82:997–1005
  25. Peavy DE, Abram JD, Frank BH, Duckworth WC. In vitro activity of biosynthetic human proinsulin: receptor binding and biologic potency of proinsulin and insulin in isolated rat adipocytes. Diabetes. 1984;33:1062–1067
  26. Marriott D, Gillece-Castro B, Gorman CM. Prohormone convertase-1 will process prorelaxin, a member of the insulin family of hormones. Mol Endocrinol. 1992;6:1441–1450
  27. Pauloin A, Tooze SA, Michelutti I, Delpal S, Ollivier-Bousquet M. The majority of clathrin coated vesicles from lactating rabbit mammary glands arise from secretory pathways. J Cell Sci. 1999;112:4089–4100
  28. Du J, Keegan BP, North WG. Key peptide processing enzymes are expressed by breast cancer cells. Cancer Lett. 2001;165:211–218
  29. Liu C, Eriste E, Sutton S, Chen J, Roland B, Kuei C, et al. Identification of relaxin-3/INSL7 as an endogenous ligand for the orphan G-protein coupled receptor GPCR135. J Biol Chem. 2003;278:50754–50764
  30. Jetten AM, Bernacki SH, Floyd EE, Saunders NA, Pieniazek J, Lotan R. Expression of a preprorelaxin-like gene during squamous differentiation of rabbit tracheobronchial epithelial cells and its suppression by retinoic acid. Cell Growth Differ. 1992;3:549–556
  31. Eddie L, Tregear G . Prorelaxin and c-peptide in sera, seminal plasma and urine. In:  MacLennan A,  Tregear G,  Bryant-Greenwood G editor. Progress in relaxin research. Singapore: Global Production Services; 1994;p. 75–84
  32. Van Cott K, Velander WH. Transgenic animals as drug factories: a new source of recombinant protein therapeutics. Expert Opin Invest Drugs. 1998;7:1683–1690

PII: S0739-7240(05)00213-4

doi: 10.1016/j.domaniend.2005.10.001

Domestic Animal Endocrinology
Volume 31, Issue 2 , Pages 173-185 , August 2006