Endocrine and cellular characteristics of corpora lutea from cows with a delayed post-ovulatory progesterone rise

The timing of the post-ovulatory progesterone rise is critical to the embryonic development and survival. The aim of this study was to determine the underlying causes of delayed post-ovulatory progesterone rises. Two groups of non-lactating dairy cows with early (n=11) or late (n=9) post-ovulatory progesterone rises were created by inducing luteolysis in the presence of either a large (>10mm) or small (<10mm) follicle, respectively. LH pulses were measured on days 4 (all cows) and 7 (n=7, early; n=5, late) (day 1=ovulation). The cows were slaughtered on day 5 (n=4 each group) or 8 (n=7, early; n=5, late). Immunohistochemical analysis for endothelial cells (von Willebrand Factor, VWF), steroidogenic cells (3β-HSD) and proliferation marker (Ki67) were performed. The basal progesterone production and LH responsiveness (0.001–100ng/ml) of dispersed luteal cells was investigated. The luteal concentrations of FGF-2 and VEGF were measured by ELISA and RIA, respectively. There were no differences in LH pulse characteristics, area of VWF staining, proliferation index, steroidogenic cell characteristics, basal or LH-stimulated progesterone production by luteal cells between cows with an early or late progesterone rise (P>0.10). However, the area of VWF staining increased from days 5 to 8, while the proliferation index decreased (P<0.05). Furthermore, the luteal cells were more responsive to LH on day 8 (P<0.01). Luteal concentrations of FGF-2 were higher on day 5 (P=0.05), while VEGF was greater on day 8 (P<0.01). In conclusion, we have clearly shown that LH support, degree of vascularization or luteal cell steroidogenic capacity were not the major factors responsible for inadequate secretion of progesterone by the developing bovine CL.