Effects of GnRH treatment on initiation of pulses of LH, LH release, and subsequent concentrations of progesterone

Progesterone is essential for establishment and maintenance of pregnancy. One proposed method to increase progesterone is administering GnRH at insemination. However, this method has resulted in conflicting results. Therefore, 2 experiments were conducted to evaluate how administering GnRH at insemination affected pulses of luteinizing hormone (LH) and subsequent progesterone. In Experiment 1, cows were allotted to 2 treatments: (1) GnRH (100μg) given approximately 12h after initiation of estrus (n=5); and (2) Control (n=5). Blood samples were collected at 15-min intervals for 6h at 12 (blood sampling period 1), 26 (blood sampling period 2), 40 (blood sampling period 3), 54 (blood sampling period 4), and 68 (blood sampling period 5) h after onset of estrus. Daily blood samples were collected for 17 d. In Experiment 2, cows were allotted into 2 treatments: GnRH administered 10 to 11h (n=10) or 14 to 15h (n=10) after onset of estrus. Daily blood samples were collected for 17 d. Cows treated with GnRH tended (P≤0.075) to have greater LH release during blood sampling period 1, tended (P=0.095) to have fewer pulses during blood sampling period 2, tended (P=0.067) to have greater concentrations of progesterone, and had an earlier (P=0.05) increase in progesterone than control cows. Cows treated with GnRH 10 to 11h after onset of estrus had greater (P=0.01) progesterone and an earlier (P=0.04) increase in progesterone than cows treated 14 to 15h. In conclusion, timing of GnRH treatment following onset of estrus influenced pulses of LH and subsequent progesterone.