Domestic Animal Endocrinology
Volume 37, Issue 4 , Pages 227-235 , November 2009

Interactions between genes involved in growth and muscularity in pigs: IGF-2, myostatin, ryanodine receptor 1, and melanocortin-4 receptor

  • A. Stinckens

      Affiliations

    • Departement of Biosystems, KULeuven, 3001 Heverlee, Belgium
  • ,
  • T. Luyten

      Affiliations

    • Departement of Biosystems, KULeuven, 3001 Heverlee, Belgium
  • ,
  • K. Van den Maagdenberg

      Affiliations

    • Leuven Food Science and Nutrition Research Centre, KaHo St. Lieven, 9000 Gent, Belgium
  • ,
  • S. Janssens

      Affiliations

    • Departement of Biosystems, KULeuven, 3001 Heverlee, Belgium
  • ,
  • S. De Smet

      Affiliations

    • Laboratory for Animal Nutrition and Animal Product Quality, Department of Animal Production, Ghent University, 9090 Melle, Belgium
  • ,
  • M. Georges

      Affiliations

    • Animal Genomics Unit, University of Liège, 4000 Liège, Belgium
  • ,
  • N. Buys

      Affiliations

    • Departement of Biosystems, KULeuven, 3001 Heverlee, Belgium
    • Corresponding Author InformationCorresponding author. Kasteelpark Arenberg 30, 3001 Heverlee, Belgium. Tel.: +32 16 321438; fax: +32 16 321994.

Received 12 March 2009 ,Revised 23 June 2009 ,Accepted 24 June 2009.

References 

  1. Fujii J, Otsu K, Zorzato F, et al. Identification of a mutation in porcine ryanodine receptor associated with malignant hyperthermia. Science. 1991;253:448–451
  2. Kim KS, Larsen N, Short T, Plastow G, Rothschild MF. A missense variant of the porcine melanocortin-4 receptor (MC4R) gene is associated with fatness, growth and feed intake traits. Mamm Genome. 2000;11:131–135
  3. Van Laere A, Nguyen M, Braunschweig M, et al. A regulatory mutation in IGF2 causes a major QTL effect on muscle growth in the pig. Nature. 2003;425:832–836
  4. Stinckens A, Luyten T, Bijttebier J, et al. Characterisation of the complete porcine MSTN gene and expression levels in pig breeds differing in muscularity. Anim Genet. 2008;39:586–596
  5. Jeon JT, Carlborg Ö, Törnsten A, et al. A paternally expressed QTL affecting skeletal and cardiac muscle mass in pigs maps to the IGF2 locus. Nat Genet. 1999;21:157–158
  6. Nezer C, Moreau L, Brouwers B, et al. An imprinted QTL with major effect on muscle mass and fat deposition maps to the IGF2 locus in pigs. Nat Genet. 1999;21:155–156
  7. Florini JR, Magri KA, Ewton DZ, James PL, Grindstaff K, Rotwein PS. ‘Spontaneous’ differentiation of skeletal myoblasts is dependent upon autocrine secretion of insulin-like growth factor-II. J Biol Chem. 1991;266:15917–15923
  8. Oksbjerg N, Gondret F, Vestergaard M. Basic principles of muscle development and growth in meat-producing mammals as affected by the insulin-like growth factor (IGF) system. Domest Anim Endocrinol. 2004;27:219–240
  9. Park HB, Carlborg Ö, Marklund S, Andersson L. Melanocortin-4 receptor (MC4R) genotypes have no major effect on fatness in a Large White×Wild Boar intercross. Anim Genet. 2002;33:155–157
  10. Houston RD, Cameron ND, Rance KA. A melanocortin-4 receptor (MC4R) polymorphism is associated with performance traits in divergently selected large white pig populations. Anim Genet. 2004;35:386–390
  11. Bruun CS, Jorgensen CB, Nielsen VH, Andersson L, Fredholm M. Evaluation of the porcine melanocortin-4 receptor (MC4R) gene as a positional candidate for a fatness QTL in a cross between Landrace and Hampshire. Anim Genet. 2006;37:359–362
  12. Stachowiak M, Szydlowski M, Obarzanek-Fojt M, Switonski M. An effect of a missense mutation in the porcine melanocortin-4 receptor (MC4R) gene on production traits in Polish pig breeds is doubtful. Anim Genet. 2006;37:55–57
  13. Kambadur R, Sharma M, Smith TPL, Bass . Mutations in myostatin (GDF8) in double-muscled Belgian Blue and Piedmontese cattle. Genome Res. 1997;7:910–915
  14. Grobet L, Martin LHR, Poncelet D, et al. A deletion in the bovine myostatin gene causes the double-muscled phenotype in cattle. Nat Genet. 1997;17:71–74
  15. McPherron AC, Lee SJ. Double muscling in cattle due to mutations in the myostatin gene. PNAS. 1997;94:12457–12461
  16. Szabó G, Dallmann G, Müller G, Patthy L, Soller M, Varga L. A deletion in the myostatin gene causes the compact (Cmpt) hypermuscular mutation in mice. Mamm Genome. 1998;9:671–672
  17. Schuelke M, Wagner KR, Stolz LE, et al. Myostatin mutation associated with gross muscle hyperthrophy in a child. New Engl J Med. 2004;350:2682–2688
  18. Clop A, Marcq F, Takeda H, et al. A mutation creating a potential illegitimate microRNA target site in the myostatin gene affects muscularity in sheep. Nat Genet. 2006;38:813–818
  19. Mosher DS, Quignon P, Bustamante CD, et al. A mutation in the myostatin gene increases muscle mass and enhances racing performance in heterozygous dogs. PloS Genet. 2007;3:e79
  20. Stinckens A, Van den Maagdenberg K, Luyten T, Georges M, De Smet S, Buys N. The RYR1g.1843C>T mutation is associated with the effect of the IGF2 intron3-g. 3072G>A mutation on muscle hypertrophy. Anim Genet. 2007;38:67–71
  21. Sambrook J, Russell DW. Molecular Cloning: A Laboratory Manual. New York, NY: Cold Spring Harbor Laboratory Press; 2001;
  22. Schmittgen TD, Zakrajsek BA. Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR. J Biochem Biophys Methods. 2000;46:69–81
  23. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ddCt method. Methods. 2001;25:402–408
  24. Kocamis H, Gahr SA, Batelli L, Hubbs AF, Killefer J. IGF-I, IGF-II, and IGF-receptor-1 transcript and IGF-II protein expression in myostatin knockout mice tissues. Muscle Nerve. 2002;26:55–63
  25. Lalani R, Bhasin S, Byhower F, et al. Myostatin and insulin-like growth factor-I and –II expression in the muscle of rats exposed to the microgravity environment of the Neurolab space shuttle flight. J Endocrinol. 2000;167:417–428
  26. Guimaraes SEF, Stahl CH, Lonergan SM, Geiger B, Rothschild MF. Myostatin promoter analysis and expression pattern in pigs. Livest Sci. 2007;112:143–150
  27. Mascarello F, Stecchini ML, Rowlerson A, Ballocchi E. Tertiary myotybes in postnatal growing pig muscle detected by their myosin isoform composition. J Anim Sci. 1992;70:1806–1813
  28. Mesires NT, Doumit ME. Satellite cell proliferation and differentiation during postnatal growth of porcine skeletal muscle. Am J Physiol Cell Physiol. 2002;282:C899–906
  29. Van den Maagdenberg K, Claeys E, Stinckens A, Buys N, De Smet S. Effect of age, muscle type, and insulin-like growth factor-II genotype on muscle proteolytic and lipolytic enzyme activities in boars. J Anim Sci. 2007;85:953–960
  30. Barnoy S, Glazer T, Kosower NS. The calpain-calpastatin system and protein degradation in fusing myoblasts. Biochim Biophys Acta. 1998;1402:52–60
  31. Dourdin N, Balcerzak D, Brustis JJ, Poussard S, Cottin P, Ducastaing A. Potential m-calpain substrates during myoblast fusion. Exp Cell Res. 1999;246:433–442
  32. Barnoy S, Glaser T, Kosower NS. The role of calpastatin (the specific calpain inhibitor) in myoblast differentiation and fusion. Biochim Biophys Res Commun. 1996;220:933–938
  33. Misquitta CM, Chen T, Grover AK. Control of protein expression through mRNA stability in calcium signalling. Cell Calcium. 2006;40:329–346
  34. Franck M, Figwer P, Godfraind C, Poirel MT, Khazzaha A, Ruchoux MM. Could the pale, soft, and exudative condition be explained by distinctive histological characteristics?. J Anim Sci. 2007;85:746–753
  35. Sharma M, Kambadur R, Matthews KG, et al. Myostatin, a transforming growth factor-β superfamily member, is expressed in heart muscle and is upregulated in cardiomyocytes after infarct. J Cell Physiol. 1999;180:1–9
  36. Morissette MR, Cook SA, Foo S, et al. Myostatin regulates cardiomyocyte growth through modulation of Akt signalling. Circ Res. 2006;99:15–24
  37. McKoy G, Bicknell KA, Patel K, Brooks G. Developmental expression of myostatin in cardiomyocytes and its effect on foetal and neonatal rat cardiomyocyte proliferation. Cardiovasc Res. 2007;74:304–312
  38. Liu Q, Yan H, Dawes NJ, Mottino GA, Frank JS, Zhu H. Insulin-like growth factor-II induces DNA synthesis in fetal vetricular myocytes in vitro. Circ Res. 1996;79:716–726

PII: S0739-7240(09)00072-1

doi: 10.1016/j.domaniend.2009.06.002

Domestic Animal Endocrinology
Volume 37, Issue 4 , Pages 227-235 , November 2009