Domestic Animal Endocrinology
Volume 38, Issue 3 , Pages 138-145 , April 2010

Presence of anti-insulin natural autoantibodies in healthy cats and its interference with immunoassay for serum insulin concentrations

  • N. Nishii

      Affiliations

    • Laboratory of Veterinary Pharmacotherapeutics, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan
    • ,
  • M. Takasu

      Affiliations

    • Laboratory of Veterinary Clinical Theriogenology, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan
    • ,
  • M. Kojima

      Affiliations

    • Shibayagi Co., Ltd., Gunma 377-0007, Japan
    • ,
  • T. Hachisu

      Affiliations

    • Shibayagi Co., Ltd., Gunma 377-0007, Japan
    • ,
  • K. Wakabayashi

      Affiliations

    • Shibayagi Co., Ltd., Gunma 377-0007, Japan
    • ,
  • A. Iwasawa

      Affiliations

    • Laboratory of Comparative Biochemistry, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan
    • ,
  • S. Maeda

      Affiliations

    • Laboratory of Veterinary Internal Medicine, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan
    • ,
  • Y. Ohba

      Affiliations

    • Laboratory of Veterinary Internal Medicine, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan
    • ,
  • H. Kitagawa

      Affiliations

    • Laboratory of Veterinary Internal Medicine, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan
    • Corresponding Author InformationCorresponding author. Laboratory of Veterinary Internal Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, Japan 501-1193 Tel.: +81 58 293 2950 fax: +81 58 293 2964.

Received 8 July 2009 ,Revised 7 September 2009 ,Accepted 7 September 2009.

References 

  1. Palmer JP, Asplin CM, Clemons P, Lyen K, Tatpati O, Raghu PK, et al. Insulin antibodies in insulin-dependent diabetics before insulin treatment. Science. 1983;222:1337–1339
  2. Reeves WG, Kelly U. Insulin antibodies induced by bovine insulin therapy. Clin Exp Immunol. 1982;50:163–170
  3. Burch HB, Clement S, Sokol MS, Landry F. Reactive hypoglycemic coma due to insulin autoimmune syndrome: case report and literature review. Am J Med. 1992;92:681–685
  4. Rendell M, Hamilton RG, Drew HM, Adkinson NF. Exacerbation of diabetes mellitus by antibodies to exogenous insulin. Am J Med Sci. 1981;282:18–26
  5. Archambeaud-Mouveroux F, Huc MC, Nadalon S, Fournier MP, Canivet B. Autoimmune insulin syndrome. Biomed Pharmacother. 1989;43:581–586
  6. Casesnoves A, Mauri M, Dominguez JR, Alfayate R, Picó AM. Influence of anti-insulin antibodies on insulin immunoassays in the autoimmune insulin syndrome. Ann Clin Biochem. 1998;35(Pt 6):768–774
  7. Sapin R. Anti-insulin antibodies in insulin immunometric assays: a still possible pitfall. Eur J Clin Chem Clin Biochem. 1997;35:365–367
  8. Lutz TA, Rand JS. Comparison of five commercial radioimmunoassay kits for the measurement of feline insulin. Res Vet Sci. 1993;55:64–69
  9. Nishii N, Takasu M, Soe OK, et al. Measurement of feline plasma insulin concentrations by use of ELISA. J Jpn Vet Med Assoc. 2007;60:290–293
  10. Thorell JI, Johansson BG. Enzymatic iodination of polypeptides with 125I to high specific activity. Biochim Biophys Acta. 1971;251:363–369
  11. Gerbitz KD, Kemmler W. Method for rapid quantitation and characterization of insulin antibodies. Clin Chem. 1978;24:890–894
  12. Rosenthal HE. A graphic method for the determination and presentation of binding parameters in a complex system. Anal Biochem. 1967;20:525–532
  13. Lacroix-Desmazes S, Kaveri SV, Mouthon L, Ayouba A, Malanchère , Coutinho A, et al. Self-reactive antibodies (natural autoantibodies) in healthy individuals. J Immunol Methods. 1998;216:117–137
  14. Adib M, Ragimbeau J, Avrameas S, Ternynck T. IgG autoantibody activity in normal mouse serum is controlled by IgM. J Immunol. 1990;145:3807–3813
  15. Kiss K, Uher F, Gergely J. A natural IgM antibody does inhibit polyclonal and antigen-specific IgM but not IgG B-cell responses. Immunol Lett. 1994;39:235–241
  16. Galili U, Clark MR, Shohet SB. Excessive binding of natural anti-alpha-galactosyl immunoglobin G to sickle erythrocytes may contribute to extravascular cell destruction. J Clin Invest. 1986;77:27–33
  17. Quintana FJ, Buzas E, Prohászka Z, Bíró A, Lpcsos K, Füst G, et al. Knock-out of the histidine decarboxylase gene modifies the repertoire of natural autoantibodies. J Autoimmun. 2004;22:297–305
  18. Mouthon L, Nobrega A, Nicolas N, Kaveri SV, Barreau C, Coutinho A, et al. Invariance and restriction toward a limited set of self-antigens characterize neonatal IgM antibody repertoires and prevail in autoreactive repertoires of healthy adults. Proc Natl Acad Sci U S A. 1995;92:3839–3843
  19. Quintana FJ, Getz G, Hed G, Doany E, Cohen IR. Cluster analysis of human autoantibody reactivities in health and in type 1 diabetes mellitus: a bio-informatic approach to immune complexity. J Autoimmun. 2003;21:65–75
  20. Quintana FJ, Cohen IR. Autoantibody patterns in diabetes-prone NOD mice and in standard C57BL/6 mice. J Autoimmun. 2001;17:191–197
  21. Dighiero G, Lymberi P, Mazié JC, Rouyre S, Butler-Browne GS, Whalen RG, et al. Murine hybridomas secreting natural monoclonal antibodies reacting with self antigens. J Immunol. 1983;131:2267–2272
  22. Bobrzynski T, Fux M, Vogel M, Stadler MB, Stadler BM, Miescher SM. A high-affinity natural autoantibody from human cord blood defines a physiologically relevant epitope on the FcepsilonRIalpha. J Immunol. 2005;175:6589–6596
  23. Vähäsalo P. Autoantibodies to insulin have similar affinity to that of antibodies to exogenous insulin but lower binding capacity. Eur J Clin Invest. 1992;22:772–776
  24. Ho LT, Wang LM, Chang TC, et al. An in vitro approach to the kinetics of insulin antibodies in diabetic patients. Zhonghua Yi Xue Za Zhi (Taipei). 1990;46:65–72
  25. Sapin R. Insulin assays: previously known and new analytical features. Clin Lab. 2003;49:113–121
  26. Shechter Y, Chang KJ, Jacobs S, Cuatrecasas P. Modulation of binding and bioactivity of insulin by anti-insulin antibody: relation to possible role of receptor self-aggregation in hormone action. Proc Natl Acad Sci U S A. 1979;76:2720–2724
  27. Keilacker H, Knospe S, Ziegler M, Whittaker J. Effect of antibody-mediated crosslinking of insulin on its bioactivity and receptor binding. Exp Clin Endocrinol. 1985;85:38–46
  28. Hall TR, Thomas JW, Padoa CJ, Torn C, Landin-Olsson M, Ortqvist E, et al. Longitudinal epitope analysis of insulin-binding antibodies in type 1 diabetes. Clin Exp Immunol. 2006;146:9–14
  29. Castaño L, Ziegler AG, Ziegler R, Shoelson S, Eisenbarth GS. Characterization of insulin autoantibodies in relatives of patients with type I diabetes. Diabetes. 1993;42:1202–1209
  30. Conlon JM. Evolution of the insulin molecule: insights into structure-activity and phylogenetic relationships. Peptides. 2001;22:1183–1193

PII: S0739-7240(09)00102-7

doi: 10.1016/j.domaniend.2009.09.001

Domestic Animal Endocrinology
Volume 38, Issue 3 , Pages 138-145 , April 2010

Article Tools

* Image Usage & Resolution