Cloning, expression analysis, and regulatory mechanisms of bovine chemerin and chemerin receptor

Song, S.-H.; Fukui, K.; Nakajima, K.; Kozakai, T.; Sasaki, S.; Roh, S.-G.; Katoh, K.

doi:10.1016/j.domaniend.2010.02.007

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Volume 39, Issue 2 , Pages 97-105, August 2010

Cloning, expression analysis, and regulatory mechanisms of bovine chemerin and chemerin receptor

S.-H. Song
- Laboratory of Animal Physiology, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai 981-8555
K. Fukui
- Department of Food Production Science, Faculty of Agriculture, Shinshu University, Nagano-ken 399-4598
K. Nakajima
- Department of Animal Production and Grassland, National Agricultural Research Center for Hokkaido Region, Toyohira-ku, Sapporo, Japan
T. Kozakai
- Department of Animal Production and Grassland, National Agricultural Research Center for Hokkaido Region, Toyohira-ku, Sapporo, Japan
S. Sasaki
- Department of Food Production Science, Faculty of Agriculture, Shinshu University, Nagano-ken 399-4598
S.-G. Roh
- Laboratory of Animal Physiology, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai 981-8555
- Address for correspondence: Laboratory of Animal Physiology, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai 981-8555, Japan; Phone and Fax: 81 22 717 6898
K. Katoh
- Laboratory of Animal Physiology, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai 981-8555

published online 20 April 2010.

Abstract

Recently, we reported that chemerin, a new adipokine, is highly expressed in the adipose tissue, up-regulated during adipocyte differentiation, and regulates adipogenesis via its own receptor in mice. The objectives of this study were to clone chemerin and its receptor from the adipose tissues of Japanese Black cattle and to investigate the expression of these genes in 16 different tissues. We compared the gene expression of chemerin and its receptor between adipocytes and stromal-vascular (S-V) cells (non-adipocytes) prepared from subcutaneous adipose tissue. In addition, we investigated the mRNA expression levels of chemerin and its receptor in bovine differentiated adipocytes. The DNA sequences of bovine chemerin and its receptor were determined, and they were found to be highly homologous to those of humans, mice, and pigs. The amino acid sequences predicted for the full-length cDNA of bovine chemerin and its receptor were also similar to those of humans, mice, and pigs, suggesting that these genes have similar functions. Bovine chemerin mRNA was highly expressed in the adipose and liver tissues, and the transcripts of chemerin receptor were widely expressed in several tissues including adipose, muscle, liver, and brain tissues. The expression of bovine chemerin mRNA was higher in adipocytes than in S-V cells prepared from adipose tissue. The transcripts of chemerin and its receptor were up-regulated during adipocyte differentiation. Treatment with tumor necrosis factor (TNF)-α (10 ng/mL) in bovine differentiated adipocytes increased the mRNA expression of chemerin and its receptor. These results indicate that chemerin, a new adipokine highly expressed in the adipocytes of bovine adipose tissue, is the TNF-α-up-regulated gene with a role in adipogenesis.

Keywords: Cattle, Chemerin, Chemerin receptor, Adipose tissue