Expression of extracellular matrix metalloproteinase inducer (EMMPRIN) and its expected roles in the bovine endometrium during gestation

Mishra, B.; Kizaki, K.; Koshi, K.; Ushizawa, K.; Takahashi, T.; Hosoe, M.; Sato, T.; Ito, A.; Hashizume, K.

doi:10.1016/j.domaniend.2011.09.004

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Volume 42, Issue 2 , Pages 63-73, February 2012

Expression of extracellular matrix metalloproteinase inducer (EMMPRIN) and its expected roles in the bovine endometrium during gestation

B. Mishra
- Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Iwate University, Morioka, Iwate 020-8550, Japan
- United Graduate School of Veterinary Science, Gifu University, Gifu City, Japan
K. Kizaki
- Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Iwate University, Morioka, Iwate 020-8550, Japan
- United Graduate School of Veterinary Science, Gifu University, Gifu City, Japan
K. Koshi
- Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Iwate University, Morioka, Iwate 020-8550, Japan
- United Graduate School of Veterinary Science, Gifu University, Gifu City, Japan
K. Ushizawa
- Department of Developmental Biology, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan
T. Takahashi
- Department of Developmental Biology, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan
M. Hosoe
- Department of Developmental Biology, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan
T. Sato
- Department of Biochemistry and Molecular Biology, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192-0392, Japan
A. Ito
- Department of Biochemistry and Molecular Biology, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192-0392, Japan
K. Hashizume
- Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Iwate University, Morioka, Iwate 020-8550, Japan
- United Graduate School of Veterinary Science, Gifu University, Gifu City, Japan
- Corresponding author at: Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Iwate University, Ueda 3-18-8, Morioka, Iwate 020-8550, Japan. Tel.: +81(8) 19621 6210; fax: +81(8) 19621 6210

Received 14 June 2011; received in revised form 8 September 2011; accepted 10 September 2011. published online 28 October 2011.

Abstract

Extracellular matrix metalloproteinase inducer (EMMPRIN) and its induced matrix metalloproteinases (MMPs) play a crucial role in tissue remodeling during the peri-implantation period. However, the role of EMMPRIN in the bovine placenta is still unclear. We have postulated that EMMPRIN might play a regulatory role in trophoblastic cell functions during gestation by itself or through the regulation of MMP expression. In this study, EMMPRIN mRNA was detected in the bovine placentome and interplacentome throughout gestation, and its expression was significantly higher in the cotyledon during late gestation. In situ hybridization showed that EMMPRIN mRNA was expressed in the caruncular epithelium and the cotyledonary epithelium, including binucleate cells. Western blot analysis detected a band representing a protein of approximately 65 kDa in the caruncular and cotyledonary tissues, and the intensity of its expression was increased in both of these tissues during late gestation. The expression levels of MMP-2 and MMP-14 in the bovine placenta were higher during late gestation, as was observed for EMMPRIN. Therefore, EMMPRIN might regulate trophoblastic cell functions, especially those of binucleate cells, through MMP expression in the bovine placenta.

Keywords: Bovine placenta , Extracellular matrix metalloproteinase inducer , EMMPRIN , Tissue remodeling , Matrix metalloproteinase , MMP